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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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Biologia
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JoVE Journal Biologia
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

DOI:
10.3791/1958-v

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09:20 min

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July 23, 2010

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, , , ,
1Department of Biochemistry, School of Medical Sciences,University of Bristol, 2Division of Immunity and Infection, School of Medicine,University of Birmingham

Capítulos

  • 00:04Título
  • 00:48Introduction
  • 01:21Attaching Cells to Coverslips
  • 02:51Subcellular Fractionation
  • 06:23Preparing Cells for Immunofluorescence Microscopy
  • 08:15Results of Subcellular Fractionation
  • 08:57Conclusion

Summary

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In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.

Tags

In Situ Subcellular FractionationAdherent Mammalian CellsNon-adherent Mammalian CellsProtein LocalizationProtein CompartmentalizationProtein Sub-populationsSpecific StructuresTranscription FactorsChromatin AssociationNuclear ScaffoldNuclear MatrixMicroscope CoverslipsProtein VisualizationAntibodiesFluorescent Fusion ProteinsFluorescence MicroscopySpecific Compartments Or StructuresProtein MappingWestern Blotting

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