Journal
/
Biologia
/
制备果蝇 S2细胞，光学显微镜
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Preparation of Drosophila S2 cells for Light Microscopy
Please note that all translations are automatically generated. Click here for the English version.

制备果蝇 S2细胞，光学显微镜

DOI:
10.3791/1982-v

•

09:39 min

•

June 03, 2010

•

, , ,
1Department of Cell Biology and Anatomy,University of Arizona (UOA)

Capítulos

  • 00:00Título
  • 00:51Introduction
  • 01:28Preparation of Glass-Bottom Dishes
  • 04:16Preparation of S2 Cells for Live Cell Microscopy
  • 05:12Fixation and Immunostaining of S2 Cells in the Glass-bottom Dish
  • 07:50Results of Microscopy of S2 Cells
  • 09:21Conclusion

Summary

Tadução automática

Tadução automática

果蝇施耐德（S2）细胞基因的发现和功能分析系统越来越受欢迎。我们的目标是描述这样一个日益重要的实验系统，使S2细胞的显微技术。

Tags

Drosophila S2 CellsLight MicroscopyExperimental SystemTechniquesLiteratureGenome Sequence DatabaseCellular ProcessesGenesMolecular ComponentsRNAiFluorescence MicroscopyLive CellsFixed CellsMediaSerum-free MediaTemperatureContainersAtmosphereSealed FlasksPhenotypes

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

电子显微镜对胚胎的制备果蝇胚胎心管

考试<em>果蝇</em幼虫气管终端细胞光镜

细胞器运输的培养<em>果蝇</em>细胞：S2细胞系和原代神经元。

在造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的活体克隆跟踪

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

生成一个“人性化”<em>果蝇</em> S2细胞敏感的驱动蛋白-5的药理抑制

果蝇的制备及心功能体内使用光学相干显微镜成像纵向（OCM）

高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备

光斑分析分析果蝇拉瓦尔光轴

使用新型机器人系统 Spotiton 进行时间解决研究的低温电子微观网格准备

Read Article