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Capítulos
- 00:04Título
- 00:46Introduction
- 01:15Acquiring Ion Mobility-Mass Spectrometry Spectrum
- 07:49Screening Experimental Conditions
- 09:45Correlating Between Drift Time Values & Cross-Sectional Areas
- 13:26Representative Results
- 15:59Conclusion
Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.
Tags
T-wave Ion Mobility-mass SpectrometryProtein Complex AnalysisIon MobilityElectric FieldCollision Cross-sectionIM SpectrometryMass SpectrometryTraveling-wave Synapt Ion Mobility Mass SpectrometerSample SeparationThree-dimensional SpectrumSpectral OverlapResolution Of Heterogeneous ComplexesDrift Time MeasurementsStructural InformationCalibration Curve
