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Cell Electrofusion Visualized with Fluorescence Microscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Cell Electrofusion Visualized with Fluorescence Microscopy

Cell Electrofusion Visualized with Fluorescence Microscopy

DOI:
10.3791/1991-v

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05:02 min

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July 01, 2010

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, ,
1Faculty of Electrical Engineering,University of Ljubljana

Capítulos

  • 00:00Título
  • 00:20Loading the Cells with Cell Trackers CMFDA and CMRA
  • 02:31Electrofusion
  • 03:39Image Acquistion and Determination of the Fusion Yield

Summary

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In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.

Tags

Cell ElectrofusionFluorescence MicroscopyHybrid CellsHuman TherapyNon-viral MethodNon-chemical MethodHigh-voltage Electric PulsesCell Plasma MembranesPermeabilityFusionLipid BilayersCytoplasmAdherence MethodCell Tracker DyesOrange CMRAGreen CMFDAFusion Yield

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