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技术成像钙^{2 +}信号在人类精子

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Techniques for Imaging Ca²⁺ Signaling in Human Sperm

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技术成像钙^{2 +}信号在人类精子

DOI:

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07:38 min

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June 16, 2010

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Katherine Nash, Linda Lefievre, Ruben Peralta-Arias, Jennifer Morris, Aduen Morales-Garcia, Tom Connolly, Sarah Costello, Jackson C. Kirkman-Brown, Stephen J. Publicover

¹School of Biosciences,University of Birmingham, ²School of Medicine,University of Birmingham, ³Centre for Human Reproductive Science,Birmingham Women’s Hospital

Capítulos

00:00Título
00:50Introduction
01:26Preparation and Staining of Sperm Cells
04:32Imaging Sperm Intracellular Ca Signals
06:20Single-cell Fluorescence Intensity Data and its Relationship to Free Calcium
07:06Conclusion

Summary

Tadução automática

刺激诱发的[Ca<sup> 2 +</sup>]：我个人的人类精子的信号进行评估。运动型细胞加载与Ca<sup> 2 +</sup>敏感的荧光染料（AM -酯法），并固定在perfusable室。时间推移荧光显微镜和细胞成像，通过灌注介质刺激。单细胞的反应（或地区）使用Excel进行离线分析。

Tags

Fluorescence Microscopy Ca2+ Signaling Human Sperm Fluorescent Dyes Loading Method Physiological Stimulation Capacitating Conditions AM Ester Cytoplasm Visible Wavelength Dyes Ratiometric Recording Advantages And Disadvantages Imaging Chamber Poly-D-lysine Coated Coverslip Perfusion Apparatus Time-lapse Acquisition Regions Of Interest Data Normalization

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