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Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
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Biologia
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JoVE Journal Biologia
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

DOI:
10.3791/200-v

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15:12 min

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May 28, 2007

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, ,
1Department of Development and Cell Biology, Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Capítulos

  • 00:19Introduction
  • 01:14Preparation
  • 01:54Collection of pupae
  • 02:36Isolating pupae head
  • 04:13Isolating brain
  • 05:57Processing isolated brain
  • 07:04Washing off enzyme solution
  • 07:58Trituration and plating
  • 12:23Imaging calcium flux in pupal neurons
  • 13:26Conclusion

Summary

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This video demonstrates the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. Views of live cultures show neurite outgrowth and imaging of calcium levels using Fura-2.

Tags

Primary Neuronal CulturesLate Stage Drosophila PupaeBrain RemovalPapain IncubationSerum-free Growth MediumMechanical DissociationAxonsDendritesRegenerationLive CulturesGAL4 LinesFluorescent MarkersWhole Cell RecordingsCholinergic SynapsesGABAergic SynapsesCalcium DynamicsFura-2 DyeGenetic ToolsPharmacological Tools

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