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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

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¹³名C₄-アスパラギン酸および ¹³C₅-グルタミン安定同位体トレーシング、LC-MS分析、およびSDH活性測定

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

¹³C₄-Aspartate and ¹³C₅-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

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¹³名C₄-アスパラギン酸および ¹³C₅-グルタミン安定同位体トレーシング、LC-MS分析、およびSDH活性測定

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

DOI:

10.3791/200017-v

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04:55 min

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May 19, 2023

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Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli²

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Tags

13C4-aspartate 13C5-glutamine Stable Isotope Tracing LC-MS Analysis SDH Activity Measurement 143B Human Osteosarcoma Cells Cell Culture Metabolite Extraction Vacuum Concentration Liquid Chromatography Mass Spectrometry

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¹³名C₄-アスパラギン酸および ¹³C₅-グルタミン安定同位体トレーシング、LC-MS分析、およびSDH活性測定

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