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High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly
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Biologia
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JoVE Journal Biologia
High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly

High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly

DOI:
10.3791/200046-v

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03:20 min

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April 28, 2023

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1National Institute of Biological Sciences, Beijing, 2Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University

Tags

High-pressure FreezingHPFFreeze-substitution FixationFSFSapphire Disc-carrier Assembly1-hexadeceneFreeze-substitution SolutionAcetoneRehydrationCentrifuge TubeTweezersLiquid NitrogenTemperature Control

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