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Конструкционный проект для трансфекции цистеин-инженерных потенциал-зависимых каналов Ca
2+
в изолированных волокнах скелетных мышц мышей
JoVE Journal
Biology
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JoVE Journal
Biology
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca
2+
Channels in Murine Isolated Skeletal Muscle Fibers
Please note that all translations are automatically generated.
Click here for the English version.
Конструкционный проект для трансфекции цистеин-инженерных потенциал-зависимых каналов Ca
2+
в изолированных волокнах скелетных мышц мышей
DOI:
10.3791/200065-v
•
01:22 min
•
June 02, 2023
•
Hugo Bibollet
,
Daniel F. Bennett
,
Martin F. Schneider
,
Erick O. Hernández-Ochoa
1
Department of Biochemistry and Molecular Biology
,
University of Maryland School of Medicine
Tags
Cysteine-engineered Voltage-gated Ca2+ Channels
Murine Isolated Skeletal Muscle Fibers
CaV1.1 CDNA Construct
EGFP-tagged Alpha Subunit
Cytomegalovirus Promoter
Site-directed Mutagenesis
S4 Membrane Extracellular Interface
GFP Observation
Ionic Current Recording
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