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Chromosome Conformation Capture (3C) for Undergraduate Research and Teaching

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Generering och rening av Chromosome Conformation Capture (3C) Bibliotek

JoVE Journal
Genética

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JoVE Journal Genética

Generation and Purification of Chromosome Conformation Capture (3C) Library

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Generering och rening av Chromosome Conformation Capture (3C) Bibliotek

Chromosome Conformation Capture (3C) for Undergraduate Research and Teaching

DOI:

10.3791/200093-v

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06:25 min

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May 12, 2023

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Acadia Joniec*¹, Joseph Leszczynski*¹, Sokhna Ndoye*¹, Jillian Sylvia*¹, Steven E. Weicksel²

¹Department of Biological and Biomedical Sciences,Bryant University, ²Center of Health and Behavioral Sciences,Bryant University

Tags

Chromosome Conformation Capture 3C Library Caenorhabditis Elegans Chromatin Cross-linking Nuclei Collection DpnII Restriction Enzyme Chromatin Digestion Chromatin Ligation T4 DNA Ligase Protein Digestion Reverse Cross-linking Proteinase K RNase A Phenol-chloroform Extraction Purification

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