/

/

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

/

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

JoVE Journal
Genética

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Genética

dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

DOI:

10.3791/200139-v

•

03:34 min

•

April 21, 2023

•

Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta, Alfonso Valencia³, Biola M. Javierre⁴

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

Tags

Keywords: DATP-Tailing Adapter Ligation PCR Amplification Scarce Cell DNA Biotin Klenow Exo-minus Ligation Buffer T4 DNA Ligase Paramagnetic Bead Purification Double-Sided Selection TLE Buffer 70% Ethanol

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations (Video) | JoVE

Lysis and Digestion of Scarce Cells (Video) | JoVE

Ligation and Decrosslinking of Scarce Cell DNA (Video) | JoVE

Sonication and Biotin Pull-Down of Scarce Cells DNA (Video) | JoVE

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

Read Article