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Stria Vascularis Microdissection for Single-Nucleus Sequencing or Immunostaining
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Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE
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Biologia
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JoVE Journal Biologia
Preparation of Stria Vascularis Single-Nucleus Suspension

Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE

Stria Vascularis Microdissection for Single-Nucleus Sequencing or Immunostaining

DOI:
10.3791/200195-v

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01:28 min

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April 21, 2023

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, , , , ,
1Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Tags

Keywords: Stria VascularisSingle-nucleus SuspensionHomogenizeDounce HomogenizerLyseFilterCentrifugeSupernatantResuspendNuclei WashResuspension BufferTrypan BlueSingle Nuclei CapturesCell DensityCell Suspension

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