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Intravital Imaging Using an Inverted Microscope to Track Skin Cell Dynamics

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Montieren der Maus auf dem Live-Imaging-Einsatz für die intravitale Bildgebung

JoVE Journal
Biologia

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JoVE Journal Biologia

Mounting the Mouse on the Live Imaging Insert for Intravital Imaging

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Montieren der Maus auf dem Live-Imaging-Einsatz für die intravitale Bildgebung

Intravital Imaging Using an Inverted Microscope to Track Skin Cell Dynamics

DOI:

10.3791/200300-v

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02:15 min

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June 30, 2023

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Michael Hamersky IV, Khushi Tekale, L. Matthew Winfree, Matthew J. M. Rowan³, Lindsey Seldin^4,5,6

¹Department of Cell Biology,Emory University School of Medicine, ²Independent scholar, ³Center for Neurodegenerative Disease,Emory University School of Medicine, ⁴Department of Dermatology,Emory University School of Medicine, ⁵Winship Cancer Institute,Emory University School of Medicine, ⁶Atlanta Veterans Affairs Medical Center

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Keywords: Intravital Imaging Mouse Live Imaging Insert Isoflurane Heat Plate Body Temperature Cover Slip Ear Clip Z-stacks Time Lapse MCherry GFP Epidermal Cells Dermal Fibroblasts

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