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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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Monstervoorbereiding, RNA-extractie, PCR-opschoning en kwantificering voor sequencing van het hele genoom van het rabiësvirus (RABV)

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

Please note that all translations are automatically generated. Click here for the English version.

Monstervoorbereiding, RNA-extractie, PCR-opschoning en kwantificering voor sequencing van het hele genoom van het rabiësvirus (RABV)

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

DOI:

10.3791/200317-v

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03:08 min

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August 18, 2023

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Criselda Bautista², Gurdeep Jaswant^3,4,5, Hollie French⁶, Kathryn Campbell, Rowan Durrant, Robert Gifford⁶, Grace S. N. Kia⁸, Brian Ogoti⁹, Katie Hampson, Kirstyn Brunker⁶

¹School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health,Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

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Sample Preparation RNA Extraction PCR Clean-up Quantification Whole Genome Sequencing Rabies Virus (RABV)Ceramic Beads Lysis Buffer Brain Sample CDNA Preparation PCR Amplification Primer Pools SPRI Beads Magnetic Rack Ethanol Wash DNA Product

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