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Imunologia e Infecção

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Staining and Imaging to Visualize Neutrophil Extracellular Traps

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組織サンプル中の好中球細胞外トラップの免疫蛍光染色(英語)

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Please note that all translations are automatically generated. Click here for the English version.

組織サンプル中の好中球細胞外トラップの免疫蛍光染色(英語)

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:

10.3791/200337-v

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04:01 min

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August 18, 2023

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Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Immunofluorescence Neutrophil Extracellular Traps Blocking Solution Donkey Serum Primary Antibodies Antibody Dilution Buffer Myeloperoxidase Citrullinated Histone H3 Neutrophil Elastase Secondary Antibodies DAPI Mounting Medium

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