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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

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Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

JoVE Journal
Biologia

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JoVE Journal Biologia

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

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Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

DOI:

10.3791/200361-v

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06:38 min

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November 03, 2023

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Tiffany M. Richardson*¹, Yasminye D. Pettway*¹, John T. Walker, Heather A. Nelson, Matthew Ishahak, Gregory Poffenberger, Radhika Aramandla, Conrad Reihsmann, Ashutosh Agarwal, Alvin C. Powers^2,4, Marcela Brissova

¹Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, ²Division of Diabetes, Endocrinology and Metabolism, Department of Medicine,Vanderbilt University Medical Center, ³Department of Biomedical Engineering,University of Miami, ⁴VA Tennessee Valley Healthcare System

Tags

Fluorescent Biosensor Hormone Profiles Human Pseudoislets Microperfusion Microchip Secretagogue Brightfield Darkfield Pseudo Eyelets Fraction Collector Environmental Chamber Confocal Microscope Efflux Pump Medium Flow

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