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Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

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Neurale stamcellen van muizen vrijgeven door stereotactische intracerebroventriculaire injectie van afgiftecocktail

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Releasing Murine Neural Stem Cells by Stereotaxic Intracerebroventricular Injection of Release Cocktail

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Neurale stamcellen van muizen vrijgeven door stereotactische intracerebroventriculaire injectie van afgiftecocktail

Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

DOI:

10.3791/200381-v

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02:25 min

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February 09, 2024

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Dimitrios Dimitrakopoulos, Chrisitna Dimitriou, Freyja McClenahan, Robin J. M. Franklin³, Ilias Kazanis

¹Laboratory of Developmental Biology, Department of Biology,University of Patras, ²Wellcome Trust-MRC Cambridge Stem Cell Institute,University of Cambridge, ³Altos Labs,Cambridge Institute of Science

Tags

Keywords: Murine Neural Stem Cells Stereotaxic Intracerebroventricular Injection Release Cocktail Buprenorphine Stereotaxic Frame Povidone Iodine Hamilton Syringe Burr Hole Dura Ependymal Layer Doublecortin Subependymal Zone

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