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Closed System Production of Adherent Cells Based on Dissolvable Microcarriers

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Preparation of the Stirred-Tank Bioreactor for Large-Scale Production of Human Mesenchymal Stem Cells

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Preparation of the Stirred-Tank Bioreactor for Large-Scale Production of Human Mesenchymal Stem Cells

Preparation of the Stirred-Tank Bioreactor for Large-Scale Production of Human Mesenchymal Stem Cells

Closed System Production of Adherent Cells Based on Dissolvable Microcarriers

DOI:

10.3791/200419-v

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01:52 min

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July 07, 2023

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Yuyang Chen*¹, Huanye Xu*², Yuanyuan Zhang*², Leilei Guo, Miaomiao Lan, Yanping Yang, Wei Liu, Xiaojun Yan, Yanan Du

¹Department of Biomedical Engineering, School of Medicine,Tsinghua University, ²Beijing CytoNiche Biotechnology Co. Ltd.

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Keywords: Bioreactor Stirred-tank Mesenchymal Stem Cells PH Probe Calibration Vessel Assembly Consumable Kit Air-tightness Check Autoclave Temperature Probe Heat Mat Bioreactor Controller Microcarriers Culture Medium

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