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Imunologia e Infecção

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Neutrophil Functional Assays for Screening Different Signaling Pathways

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Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production

Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE

Neutrophil Functional Assays for Screening Different Signaling Pathways

DOI:

10.3791/200484-v

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02:59 min

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February 09, 2024

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Isabelle Souza Luz, Raquel Takaya, Daiane Gonzaga Ribeiro, Natanael Sales Silva, Lucas Fontes, Mariana S. Castro, Wagner Fontes

¹Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology,University of Brasilia, ²Institute of Physics,University of Brasilia

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Keywords: NBT Assay ROS Production Polymorphonuclear Neutrophils DMSO HBSS PMA FMLP NBT Slide Test NBT Spectrophotometry Assay Formazan Deposits SDS Sonication Absorbance Measurement

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