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Cargo Loading onto Kinesin Powered Molecular Shuttles
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JoVE Journal Biologia
Cargo Loading onto Kinesin Powered Molecular Shuttles

Cargo Loading onto Kinesin Powered Molecular Shuttles

DOI:
10.3791/2006-v

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09:00 min

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November 03, 2010

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, ,
1Department of Materials Science and Engineering,University of Florida, 2Department of Biomedical Engineering,Columbia University

Capítulos

  • 00:00Título
  • 01:00Introduction
  • 01:28Assay Preparation
  • 03:36Flow-cell Construction
  • 04:23Inverted Assay Assembly
  • 06:20Microscopy
  • 07:44Representative Results
  • 08:30Conclusion

Summary

Tadução automática

Tadução automática

Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.

Tags

Cargo LoadingKinesinMolecular ShuttlesMicrotubulesIntracellular TransportATP MoleculesSynthetic EnvironmentsBiotinStreptavidinMotility AssayCaseinTubulinRhodamine-labeled Streptavidin

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