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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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Ферментативная очистка клеток микроглии, выделенных из спинного мозга мышей на основе либеразы

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord

Please note that all translations are automatically generated. Click here for the English version.

Ферментативная очистка клеток микроглии, выделенных из спинного мозга мышей на основе либеразы

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:

10.3791/200616-v

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02:42 min

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September 22, 2023

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Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

Tags

Microglia Isolation Spinal Cord Enzymatic Digestion Liberase Density Gradient Cell Purification FACS Analysis

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