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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE
 

Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

Stable Gene Integration in Chlorella vulgaris Using Agrobacterium tumefaciens

Article DOI: 10.3791/200638-v • 04:02 min • October 27th, 2023
October 27th, 2023 •
1, 1, 1, 1
1Department of Chemical Engineering, University of Waterloo

Tags

Agrobacterium Tumefaciens-mediated Transformation Genetic Engineering Green Microalgae Chlorella Vulgaris AMT Protocol Reporter Gene Green Fluorescent Protein (mGFP5) Antibiotic Resistance Marker Hygromycin B Mutants Selection Tris-Acetate-Phosphate (TAP) Media Stable Transformation T-DNA Cassette Transgenic Colonies PCAMBIA1302 Plant Expression Vector
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