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Stable Gene Integration in Chlorella vulgaris Using Agrobacterium tumefaciens

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

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Bioengenharia

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JoVE Journal Bioengenharia

Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (cPCR)

Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

Stable Gene Integration in Chlorella vulgaris Using Agrobacterium tumefaciens

DOI:

10.3791/200638-v

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04:02 min

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October 27, 2023

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M. Reza Roushan, Chuchi Chen, Parisa Ahmadi, Valerie C. A. Ward

¹Department of Chemical Engineering,University of Waterloo

Tags

Agrobacterium-mediated Transformation Chlorella Vulgaris Colony PCR (cPCR)Gene Integration Log Phase Culture TAP Agar Supplemented LB Broth Agrobacterium Tumefaciens Induction Medium Selective Media Tetracycline DNA Agarose Gel

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

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