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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

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Flowcytometrische analyse van mitofagie in β-cellen van de pancreas van muizen met behulp van MtPhagy-kleurstof

JoVE Journal
Biologia

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JoVE Journal Biologia

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye

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Flowcytometrische analyse van mitofagie in β-cellen van de pancreas van muizen met behulp van MtPhagy-kleurstof

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

DOI:

10.3791/200661-v

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02:37 min

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September 15, 2023

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Elena Levi-D’Ancona², Vaibhav Sidarala, Scott A. Soleimanpour^3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

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Keywords: Flow Cytometry Mitophagy Pancreatic Beta Cells MtPhagy Dye TMRE Fluozin-3-AM DAPI Cell Suspension Islet Isolation Fluorescence Gating Quantification

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