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Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

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間葉系幹細胞サンプル調製、フローサイトメトリー解析、セルソーティング

JoVE Journal
Biologia

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JoVE Journal Biologia

Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting

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間葉系幹細胞サンプル調製、フローサイトメトリー解析、セルソーティング

Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

DOI:

10.3791/200704-v

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05:25 min

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November 10, 2023

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Ayona Gupta*¹, Risani Mukhopadhyay*¹, Himanshi Khandelwal, Narendra Nala, Uttara Chakraborty

¹Manipal Institute of Regenerative Medicine, Bengaluru,Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit,Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

Tags

Keywords: Mesenchymal Stem Cell Flow Cytometric Analysis Cell Sorting Sample Preparation Fax Tubes Cell And Antibody Suspension Staining Buffer FBS 10% MSC Media DAPI Compensation Matrix Single Stain Compensation Tubes Acquisition Dashboard Gating Strategy Collection Plate Single Cell Sort Purity Mask Collection Device

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