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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
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Isolering af Limbal Niche Cells (LNCs) fra Limbus Tissue
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JoVE Journal Medicina
Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue

Isolering af Limbal Niche Cells (LNCs) fra Limbus Tissue

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

DOI:
10.3791/200727-v

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05:24 min

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October 27, 2023

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, , , , , , , , , ,
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Institute of Medicine Nursing,Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

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Limbal Niche CellsLNCsCollagenase AMatrigelBasement Membrane MatrixMESCMTrypsin-EDTAKnockout SerumCell IsolationCell CultureCorneaSterile Technique

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