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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
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Isolatie van limbale nichecellen (LNC's) uit limbusweefsel
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Medicina
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JoVE Journal Medicina
Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue
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Isolatie van limbale nichecellen (LNC's) uit limbusweefsel

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

DOI:
10.3791/200727-v

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05:24 min

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October 27, 2023

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, , , , , , , , , ,
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Institute of Medicine Nursing,Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

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Limbal Niche CellsLNCsCollagenase AMatrigelBasement Membrane MatrixMESCMTrypsin-EDTAKnockout SerumCell IsolationCell CultureCorneaSterile Technique

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