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High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
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SWITCH - H2O2 - Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique (Video) | JoVE
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Neurociência
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JoVE Journal Neurociência
SWITCH – H2O2 – Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique

SWITCH - H2O2 - Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique (Video) | JoVE

High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

DOI:
10.3791/200806-v

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02:57 min

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January 26, 2024

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, , , , , , , ,
1European Laboratory for Non-linear Spectroscopy (LENS),University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine,University of Florence, 3Department of Physics,University of Pisa, 4National Research Council – National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy,University of Florence, 6Department of Biology,University of Florence

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Keywords: Antigen RetrievalSWITCHH2O2TDEFormalin-fixedParaffin-embeddedBrain SectionsSwitch-off SolutionSwitch-on SolutionPBSInactivation SolutionClearing SolutionPBSTHydrogen PeroxideAntigen Retrieval SolutionPrimary AntibodySecondary AntibodyRefractive Index MatchingTDE

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