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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

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Acquisition et vérification de pièges extracellulaires à neutrophiles (TNE) chez le rat

JoVE Journal
Biologia

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JoVE Journal Biologia

Acquisition and Verification of Rat Neutrophil Extracellular Traps (NETs)

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Acquisition et vérification de pièges extracellulaires à neutrophiles (TNE) chez le rat

Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

DOI:

10.3791/200811-v

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02:35 min

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April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

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Rat Neutrophil Neutrophil Extracellular Traps (NETs)PMA DNASE1 HBSS 4% PFA Triton X-100 Normal Donkey Serum Anti-rat Antibodies Secondary Antibodies DAPI

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