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Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

JoVE Journal
Medicina

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JoVE Journal Medicina

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

DOI:

10.3791/200823-v

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03:06 min

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November 03, 2023

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Xinyue Bai*^{1,2,3,4,5,6,7}, Ting Zhang*^{1,2,3,4,5,6,7}, Xinyue Zhu^2,3,4,5,6,7, Xianyu Huang^2,3,4,5,6,7, Haiyun Liu^2,3,4,5,6,7, Xiaoyan Ding, Mei Jiang^2,3,4,5,6,7, Xiaodong Sun^2,3,4,5,6,7

¹Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences

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Stem Cells Retinal Pigment Epithelial Cells Cryopreservation Cell Banking 5-ethynyl-2′-deoxyuridine Cell Viability Cell Recovery Rate Differentiated Cells

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