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ヒト胚性幹細胞由来網膜色素上皮細胞の解離と最適な凍結保存段階の決定

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Medicina

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JoVE Journal Medicina

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

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ヒト胚性幹細胞由来網膜色素上皮細胞の解離と最適な凍結保存段階の決定

DOI:

10.3791/200823-v

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03:06 min

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November 03, 2023

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Xinyue Bai*^{1,2,3,4,5,6,7}, Ting Zhang*^{1,2,3,4,5,6,7}, Xinyue Zhu^2,3,4,5,6,7, Xianyu Huang^2,3,4,5,6,7, Haiyun Liu^2,3,4,5,6,7, Xiaoyan Ding, Mei Jiang^2,3,4,5,6,7, Xiaodong Sun^2,3,4,5,6,7

¹Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences

Tags

Stem Cells Retinal Pigment Epithelial Cells Cryopreservation Cell Banking 5-ethynyl-2′-deoxyuridine Cell Viability Cell Recovery Rate Differentiated Cells

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ヒト胚性幹細胞由来網膜色素上皮細胞の解離と最適な凍結保存段階の決定

最適化された凍結保存によるヒト胚性幹細胞由来網膜色素上皮細胞の保存

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