Journal
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Biologia do Desenvolvimento
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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
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从斑马鱼幼虫中分离和解离肠道，以富集肠道细胞的FACS
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells
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从斑马鱼幼虫中分离和解离肠道，以富集肠道细胞的FACS

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:
10.3791/200860-v

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02:37 min

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November 10, 2023

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, , , , , ,
1Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, 2Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, 3Department of Hematology,Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Tags

GutZebrafish LarvaeFACSCell IsolationDissociationPapainAgarose PlateHEPESE3 MediumDissectionIntestineCell StrainerCentrifugationDAPICell Labeling

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