/

/

Transcriptome Profiling for High-Throughput Drug Screening

/

CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing (Video) | JoVE

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

cDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing

CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing (Video) | JoVE

Transcriptome Profiling for High-Throughput Drug Screening

DOI:

10.3791/200935-v

•

02:34 min

•

February 23, 2024

•

Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

Tags

Keywords: CDNA Library Preparation Drug-treated PBMC Lysate MRNA Sequencing Reverse Transcription Pre-amplification CDNA Cleanup Magnetic Purification Beads Tagmentation Enrichment PCR

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Cost-Effective Transcriptomic Drug Screening â€” Unlocking New Targets (Video) | JoVE

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE

CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing (Video) | JoVE

MRNA Sequencing to Understand the Cellular Effect of Drug Treatment (Video) | JoVE

Read Article