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Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

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Diferenciação in vitro de células-tronco pluripotentes humanas em células pancreáticas produtoras de insulina

JoVE Journal
Biologia

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JoVE Journal Biologia

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells

Diferenciação in vitro de células-tronco pluripotentes humanas em células pancreáticas produtoras de insulina

Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

DOI:

10.3791/200998-v

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05:06 min

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February 02, 2024

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Ines Cherkaoui, Qian Du, Dieter Egli, Shivani Misra³, Guy A. Rutter^4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

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In Vitro Human Pluripotent Stem Cells Insulin-producing Pancreatic Cells Cell Passage Coating Solution Dissociation Solution Trypan Blue Hemocytometer Rock Inhibitor Basal Medium Supplements Washing Medium Day One Medium Pancreatic Progenitor Medium Dissociation Buffer Microwells Anti-Adherence Rinsing Solution

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