Journal
/
Biologia
/
Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
/
Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells
Please note that all translations are automatically generated. Click here for the English version.

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:
10.3791/201002-v

•

04:10 min

•

June 23, 2023

•

, , , , , , , ,
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, 4Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

TransfectionHeLa CellsCENP-E KnockoutPlasmidTrypsin-EDTACell PassageReduced Serum MediumTransfection ReagentFluorescence MicroscopyCell CountingSerial DilutionPhenotype-based ScreeningDNA ExtractionPCRLigationTransformationSanger SequencingWild-typeMutant

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/201002?article_type=v

Erzeugung von Zentromer-assoziierten Protein-E CENP-E-/- Knockout-Zelllinien mit dem CRISPR/Cas9-System
check_url/pt/201002?article_type=v

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells
check_url/pt/201002?article_type=v

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells
check_url/pt/201002?article_type=v

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Read Article