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Biologia
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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
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Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:
10.3791/201003-v

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03:07 min

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June 23, 2023

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, , , , , , , ,
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, 4Medical Research Center,Fujian Maternity and Child Health Hospital

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Keywords: Immunofluorescence StainingConfocal MicroscopyCRISPR/Cas9CENP-EHeLa CellsCell FixationCell PermeabilizationPrimary AntibodySecondary AntibodyDAPIChromosome MisalignmentMetaphaseInterphaseProphaseAnaphaseTelophase

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