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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

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Forberedelse af G1-synkroniserede celler til måling af ssDNA-foci dannelse ved genotoksisk stress

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Preparation of G1 Synchronized Cells to Measure ssDNA Foci Formation upon Genotoxic Stress

Forberedelse af G1-synkroniserede celler til måling af ssDNA-foci dannelse ved genotoksisk stress

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

DOI:

10.3791/201062-v

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07:11 min

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December 22, 2023

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Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

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Here Are The Keywords From The Given Text: – G1 Synchronized Cells – SsDNA Foci Formation – Genotoxic Stress – Cell Synchronization – DNA Damage – DNA Repair

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