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A Detailed Guide for Preparing Hard Palm Seeds for MALDI-IMS Analysis

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Seed Sectioning Protocol and Matrix Deposition Technique for MALDI-IMS Analysis of Hard Palm Seeds

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Seed Sectioning Protocol and Matrix Deposition Technique for MALDI-IMS Analysis of Hard Palm Seeds

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Seed Sectioning Protocol and Matrix Deposition Technique for MALDI-IMS Analysis of Hard Palm Seeds

A Detailed Guide for Preparing Hard Palm Seeds for MALDI-IMS Analysis

DOI:

10.3791/201244-v

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02:23 min

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June 30, 2023

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Gabriel R. Martins*^1,2, Felipe L. Brum*^1,2,3, Davi Marconi Miranda Carvalho da Silva*^1,2, Livia C. Barbosa, Ronaldo Mohana-Borges, Ayla Sant’Ana da Silva²

¹Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

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Seed Sectioning Matrix Deposition MALDI-IMS Hard Palm Seeds Euterpe Precatoria Euterpe Edulis Cryostat Gelatin Embedding ITO Slide DHB Matrix APCI XY Motion Platform

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