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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei
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Probenvorbereitung für die Durchflusszytometrie zur Messung der pHluorin2-Fluoreszenz in lebenden Trypanosoma brucei
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei
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Probenvorbereitung für die Durchflusszytometrie zur Messung der pHluorin2-Fluoreszenz in lebenden Trypanosoma brucei

Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

DOI:
10.3791/201368-v

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00:54 min

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January 19, 2024

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, , , , , ,
1Department Chemistry and Biochemistry,Brigham Young University, 2Eukaryotic Pathogens Innovation Center,Clemson University, 3Department of Genetics and Biochemistry,Clemson University, 4Department of Physics and Astronomy,Clemson University

Tags

Keywords: Flow CytometryPHluorin2Trypanosoma BruceiSample PreparationTetracyclineDoxycyclineCell CultureCentrifugationPBSGlucosePropidium IodideThiazole RedLive/dead Staining

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