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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

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Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

JoVE Journal
Biologia

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JoVE Journal Biologia

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

DOI:

10.3791/201503-v

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01:14 min

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January 05, 2024

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Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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Cell Lysis HEK293 Cells Protein Sample Preparation Immunoprecipitation Transfection Protein Lysis Buffer Protease Inhibitors Cell Scraper Centrifugation Protein Concentration Protein Quantification

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