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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

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Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

JoVE Journal
Biologia

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JoVE Journal Biologia

Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

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Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

DOI:

10.3791/201504-v

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01:17 min

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January 05, 2024

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Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Tags

Keywords: Protein G Gel Protein Complex Epitope Tag Affinity Gel HEK293 Cells Transfection Protein Purification Sample Preparation Centrifugation Supernatant Input Sample Sample Buffer Heating

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