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Simplified and Efficient AAV Production Using HEK293 Suspension Culture

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Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

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Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

Simplified and Efficient AAV Production Using HEK293 Suspension Culture

DOI:

10.3791/201531-v

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03:36 min

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April 26, 2024

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Kimberly L. Pietersz, Paul J.H. Nijhuis, Matthijs H.M. Klunder, Joëlle van den Herik, Barbara Hobo, Fred de Winter, Joost Verhaagen²

¹Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), ²Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

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Keywords: Iodixanol Gradient HEK293 Cell Lysates Adeno-associated Virus Production PEG Precipitation DNase I Treatment Ultracentrifugation Virus Extraction Buffer Exchange Centrifugal Filtration

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