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Biochemistry
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הכנת תאי HASM לזיהוי cAMP באמצעות מיקרוסקופ פלואורסצנטי הפוך
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy
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הכנת תאי HASM לזיהוי cAMP באמצעות מיקרוסקופ פלואורסצנטי הפוך
DOI:
10.3791/201671-v
•
02:14 min
•
March 22, 2024
•
Isabella Cattani-Cavalieri
,
Jordyn Margolis
,
Camelia Anicolaesei
,
Francisco J. Nuñez
,
Rennolds S. Ostrom
1
Department of Biomedical and Pharmaceutical Sciences
,
Chapman University School of Pharmacy
Tags
CAMP Biosensor
CADDis
Epac2
Fluorescent Protein
CAMP Dynamics
Real-time Measurement
HEK-293 Cells
HASM Cells
96-well Plate
Fluorescent Microscopy
High-throughput Screening
CAMP Signaling
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