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Imunologia e Infecção
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Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

DOI:
10.3791/2017-v

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08:39 min

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June 01, 2010

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,
1Center for Vaccine Biology and Immunology,University of Rochester

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 01:31Isolation of Human T Lymphocytes
  • 02:57Culture of Human T Lymphocytes
  • 05:04In Vitro Lymphocyte Migration Assay
  • 06:15Capturing an Image Sequence Using NIS Elements Software
  • 07:20Representative Results
  • 08:12Conclusion

Summary

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T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.

Tags

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

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