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DNA的稳定同位素探测（DNA的SIP）
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Biologia
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JoVE Journal Biologia
DNA Stable-Isotope Probing (DNA-SIP)
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DNA的稳定同位素探测（DNA的SIP）

DOI:
10.3791/2027-v

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14:57 min

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August 02, 2010

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,
1Department of Biology,University of Waterloo

Capítulos

  • 00:04Título
  • 01:07Introduction
  • 02:11Sample Incubation and DNA Extraction
  • 03:47Gradient Solns for Ultracentrifugation
  • 05:26Ultracentrifugation
  • 07:49Gradient Fractionation
  • 09:46DNA Precipitation
  • 11:22Representative Results
  • 14:04Conclusion

Summary

Tadução automática

Tadução automática

DNA的稳定同位素探测，识别和特性的微生物能够利用特定的基板活跃的社区是一个独立的种植方法。基板同化富集重同位素的信息纳入到微生物生物量的标记原子。密度梯度超速离心检索下游分子生物学分析的DNA标记。

Tags

DNA-SIPStable-isotope ProbingMicroorganismsCarbon SubstratesNutrientsCellular BiomassCultivation-independent TechniqueMetabolic FunctionTerrestrial EnvironmentsAquatic EnvironmentsStable-isotope Labelled CompoundsNucleic AcidDensity Gradient UltracentrifugationGradient FractionationDNA PurificationCesium ChlorideMolecular CharacterizationFingerprintingMicroarraysClone LibrariesMetagenomics

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