Journal
/
Biologia
/
Microdissection of Zebrafish Embryonic Eye Tissues
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Microdissection of Zebrafish Embryonic Eye Tissues

Microdissection of Zebrafish Embryonic Eye Tissues

DOI:
10.3791/2028-v

•

09:01 min

•

June 27, 2010

•

,
1Department of Biological Sciences,Purdue University

Capítulos

  • 00:00Título
  • 01:03Introduction
  • 01:36Materials and Preparation for Microdissection
  • 04:14Brain Removal, Eye Exposure and Retinal Dissection
  • 05:57RPE-Attached Retinal Dissection
  • 07:12Dissection Integrity and RNA Expression Profiles of Whole Retina and RPE
  • 08:10Conclusion

Summary

Tadução automática

Tadução automática

This article describes an approach to microdissect zebrafish retinas with and without retinal pigment epithelium attached, from one to three days postfertilization embryos.

Tags

ZebrafishMicrodissectionEye DevelopmentGenetic ArchitectureGene Expression ProfilingRetinaRetinal Pigment Epithelium (RPE)Microdissection TechniqueEye Morphogenesis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Laser Capture Microdissection of Mammalian Tissue

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis

Microdissection of Black Widow Spider Silk-producing Glands

Immunostaining of Dissected Zebrafish Embryonic Heart

A System for ex vivo Culturing of Embryonic Pancreas

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

Read Article