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Capítulos
- 00:04Microfabrication of channels
- 00:16Mask
- 00:55Cleaning and baking the wafer
- 02:11Coating the wafer
- 04:13Soft baking the wafer
- 04:47UV exposure
- 06:14Developing the wafer
- 08:19Application of PDMS to produce channels
- 12:44Experiment 1: Investigating the chemotactic response of marine micr
- 13:44Adding organisms and substrates to glass syringes
- 14:39Assembling the microfluidics system on the microscope stage
- 15:53Observation under microscope
- 16:37Removing bubbles from channel
- 17:59Visualization of nutrient gradient and microbes
- 18:42Measurement of chemotaxis
- 19:47Experiemnt 2: Investigating the effects of shear on marine bacteria
De fabricage van microfluïdische kanalen en de uitvoering daarvan in experimenten voor het bestuderen van de chemotactische foerageergedrag van mariene microben in een fragmentarische voedingsstof zeegezicht en het zwemgedrag van de bacteriën in shear stroming worden beschreven.
Tags
Chemotactic ResponseMarine Micro-organismsMicro-scale Nutrient LayersPlanktonic MicrobesResource PatchesOceanic TrophodynamicsBiogeochemical FluxPhysical ForcesMolecular DiffusionTurbulent ShearNutrient PatchesBacteria LocationMethodological LimitationsMicrobial BehaviourPatchy HabitatsSmall-scale Flow ConditionsSoft Lithographic Fabrication TechniquesMicrofluidic DevicesMicroscale Nutrient PatchesOceanic ProcessesMicroscale VorticesShear RatesMicrobial SwimmingChemotactic BehaviourHeterogeneous SeascapeEpifluorescence MicroscopyPhase Contrast Microscopy
