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IP-FCM: Immunoprecipitation Detected by Flow Cytometry
JoVE Journal
Biologia
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JoVE Journal Biologia
IP-FCM: Immunoprecipitation Detected by Flow Cytometry
DOI:

12:17 min

December 02, 2010

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Capítulos

  • 00:08Título
  • 00:58Introduction
  • 01:31Coupling of mAb to Beads
  • 04:37Post-nuclear Lysate Preparation and IP
  • 07:07Probing Bead-captured Protein
  • 09:11FCM Acquisition
  • 11:02Representative Histogram
  • 11:37Conclusion

Summary

Tadução automática

The IP-FCM method is presented, which allows a sensitive, robust, biochemical assessment of native protein-protein interactions, without requiring genetic engineering or large sample sizes.

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