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Biologia
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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
JoVE Journal
Biologia
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JoVE Journal Biologia
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

DOI:
10.3791/2079-v

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15:55 min

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August 04, 2010

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, , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Department of Medicine and Genetics,Harvard Medical School

Capítulos

  • 00:04Título
  • 00:58Introduction
  • 01:29Microinjection Preparation
  • 03:22Microinjection
  • 08:00Fixation and Immunofluorescence
  • 10:02Plastic Embedding
  • 12:10Sectioning
  • 12:10Visualizing Nephrotoxic Kidney Damage
  • 14:54Conclusion

Summary

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We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.

Tags

Intravenous MicroinjectionsZebrafish LarvaeAcute Kidney InjuryGentamicinNephrotoxicantBloodstreamFish Per HourExperimental StudiesAngiographyProtocolKidney FunctionFixationImmunohistochemistryKidney Tubule MarkerPlastic EmbeddingSectioning

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