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斑马鱼幼虫的研究急性肾损伤的静脉Microinjections
JoVE Journal
Biologia
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JoVE Journal Biologia
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
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斑马鱼幼虫的研究急性肾损伤的静脉Microinjections

DOI:
10.3791/2079-v

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15:55 min

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August 04, 2010

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, , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Department of Medicine and Genetics,Harvard Medical School

Capítulos

  • 00:04Título
  • 00:58Introduction
  • 01:29Microinjection Preparation
  • 03:22Microinjection
  • 08:00Fixation and Immunofluorescence
  • 10:02Plastic Embedding
  • 12:10Sectioning
  • 12:10Visualizing Nephrotoxic Kidney Damage
  • 14:54Conclusion

Summary

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我们描述后fetilization（DPF）的斑马鱼幼虫诱发急性肾损伤（AKI）2天氨基糖甙类抗生素，庆大霉素，显微注射技术。我们还描述了一个整装免疫组织化学方法，塑料包埋，切片和斑马鱼幼虫形象化的AKI的介导的损伤。

Tags

Intravenous MicroinjectionsZebrafish LarvaeAcute Kidney InjuryGentamicinNephrotoxicantBloodstreamFish Per HourExperimental StudiesAngiographyProtocolKidney FunctionFixationImmunohistochemistryKidney Tubule MarkerPlastic EmbeddingSectioning

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