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RhoC的GTP酶激活法
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JoVE Journal Biologia
RhoC GTPase Activation Assay
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RhoC的GTP酶激活法

DOI:
10.3791/2083-v

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09:58 min

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August 22, 2010

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, ,
1Department of Biological Sciences, The Center for Translational Cancer Research,University of Delaware

Capítulos

  • 00:00Título
  • 00:48Introduction
  • 01:25Preparation of GST-fusion Protein
  • 04:41GST-RBD Pull Down Assay
  • 09:01Representative Results for a RhoC GTPase Activation Assay
  • 09:34Conclusion

Summary

Tadução automática

Tadução automática

该协议利用上拉下来检测，以确定细胞内的活动中RhoC GTP酶的水平。

Tags

RhoC GTPaseRhoA GTPaseActivation AssayHomologyReagentsTechniquesMetastatic Cancer CellsRho Activation AssayGST-Rho Binding Domain Fusion ProteinActive RhoC GTPaseTotal ProteinGTP BoundGDP BoundCommercial VersionsCommercial KitsRhoC-specific Antibody

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